Time to test for mites!! Checking drone brood for Varroa mites is the best testing method, in my humble opinion. There is so much room for error when you do an alcohol wash. With an alcohol wash, you are gathering 300 bees out of a colony containing upwards of 50,000 individuals, and hope that you are getting a sample that is representative of the colony as a whole. Considering 80 to 90% of the mites are under the brood cappings greatly increases your chance of error. Sugar shakes are even worse
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