A day before President Donald Trump signed an executive order banning transgender athletes from women’s sports, three University of Pennsylvania graduates sued their alma mater over the inclusion of a transgender student in a women’s swimming competition.

Three former athletes at the University of Pennsylvania women’s swimming program sued the school, the Ivy League Council of Presidents and athletics organizations Tuesday, claiming they violated federal law to allow transgender athlete Lia Thomas to compete against them.

Three former University of Pennsylvania swimmers have reportedly filed a lawsuit against the school and other organizations for allegedly violating Title IX by allowing Lia Thomas, a transgender woman, to compete in the 2022 Women’s Ivy League Swimming and Diving Championships.